Thermo Scientific™ MboI

The MboI restriction enzyme recognizes ^GATC sites and cuts best at 37°C in R buffer (Isoschizomers: BfuCI, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, NdeII, Sau3AIm).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER0811

MBOI 10U/UL 300U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Product Code. 10611241

Quantity Price
1 £ 37.6 / 300 units
EU Stock 22
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Description and Specification

Specification

Product Name MboI
Concentration 10U/µL
Units 300U

5'..▵G A T C ...3'

3'.. C T A G▵...5'

Conditions for 100% Activity:

  • 1X Buffer R: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • MboI is supplied in:10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with MboI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: completely overlaps —blocked
  • Dcm: never overlaps —no effect
  • CpG: may overlap —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: may overlap —blocked

Note:

Assayed using lambda DNA (dam) (#SD0021). MboI is blocked by overlappingdam methylation. To avoiddam methylation, use adam,dcm- strain such as GM2163 (#M0099). MboI, Bsp143I and DpnI all recognize the same sequence but have different methylation sensitivities and cleavage sites.