Thermo Scientific™ Maxima™ SYBR™ Green/Fluorescein 2X qPCR Master Mix

Optimize qPCR with SYBR Green chemistry using standard cycling protocols with these ready-to-use qPCR master mixes

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: K0241

X2 qPCR, Maxima(R) SYBR green/Fluorescein qPCR

UNSPSC: 41106300

Code: 88

Additional Details:
Additional Details: Weight: 0.03000kg



Product Code. 11833923

Quantity Price
1 £ 132.0 / 200 reactions
EU Stock 12
Add to basket


Description and Specification

Specification

For Use With (Application) Real-time PCR and RT-qPCR using SYBR Green dye on Bio-Rad iCycler iQ, MyiQ or iQ5 machines.
No. of Reactions 200 reactions of 25 ┬Ál
Quantity 2 x 1.25mL

The master mix includes Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. It contains SYBR Green I dye and is supplemented with fluorescein passive reference dye. Only template and primers need to be added.Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes. dUTP is included in the mix for optional carryover contamination control using uracil DNA glycosylase (UDG). The use of Maxima SYBR Green/Fluorescein qPCR Master Mix (2X) in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.
  • Specificity—Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity—detects low copy number targets
  • Fluorescein—supplemented with fluorescein passive reference dye for use with Bio-Rad iCycler iQ, MyiQ or iQ5
  • Wide linear range—accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience—ready-to-use 2X master mix

Recommended for:

Real-time PCR and RT-qPCR using SYBR Green dye on Bio-Rad iCycler iQ, MyiQ or iQ5 machines.