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Maxima Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

Brand:  Thermo Scientific™ K0251

136.67 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : 88

Additional Details : Weight : 2.44490kg

 View more versions of this product

Product Code. 11853923

  • £205.00 / 200 reactions
Estimated Shipment: 08-04-2024
to see stock.

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Description

Description

The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most real-time thermal cyclers.

  • Specificity - Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity - detects low copy number targets
  • Wide linear range - accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience - ready-to-use 2X master mix
Specifications

Specifications

Contains:
• 2 x 1.25 mL Maxima SYBR Green qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 200 x 25 μL reactions.
• 50 μL ROX solution, 50 μM
• 2 x 1.25 mL Nuclease-Free Water

Store at -20°C.
Tube
qPCR
Standard
Maxima
200 Reactions of 25 μL
2X
DNA, dsDNA
SYBR
High
Taq DNA Polymerase
BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Real Time PCR SYBR Master Mix
Gene Expression
200 reactions
2 x 1.25 mL
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For Research Use Only. Not for use in diagnostic procedures.