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Long PCR Enzyme Mix Not available in the USA

A unique enzyme blend for long and tricky PCR applications.

Brand:  Thermo Scientific K0182

UNSPSC : 41106300

Code : 88

Additional Details : Weight : 0.05000kg

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Product Code. 10470280

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance
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Disclaimers:

This product is licensed under one U.S. Patent No. 5,436,149 or corresponding foreign patents owned by Takara Bio, Inc.


Specifications

Description

The two enzymes synergistically generate long PCR products with greater yield and fidelity than Taq DNA Polymerase alone. The fidelity of PCR with this enzyme mix is three times higher than with Taq DNA Polymerase. The ratio of enzymes in the Long PCR Enzyme Mix is optimized for generation of very long amplicons: up to 47kb with viral DNA and up to 21kb with genomic DNA templates (see Fig. 1). The specially formulated Long PCR Buffer protects DNA from depurination and nicking during long thermal cycling. The products generated with the Long PCR Enzyme Mix are mostly 3'-dA tailed.Long PCR Enzyme Mix is also used for efficient amplification of GC-rich DNA regions (see Fig. 2).
  • Unique blend of two thermostable DNA polymerases
  • Long PCR products:—up to 47kb with viral DNA as template,—up to 21kb with genomic DNA as template
  • Ideal for GC-rich templates up to 85%
  • Fidelity is three-times higher than with Taq DNA polymerase (see Fig. 1)
  • High yield

Recommended for:

Long range PCR; PCR from GC-rich or difficult templates; Generation of template for TA cloning; RT-PCR.

Description & Specifications

Specifications

Long PCR Enzyme Mix
Long range PCR; PCR from GC-rich or difficult templates; Generation of template for TA cloning; RT-PCR.
500U
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