Long PCR Enzyme Mix Not available in the USA

A unique enzyme blend for long and tricky PCR applications.

Overview
Brand: Thermo Scientific

Manufacturer Part Number: K0181

LONG PCR ENZYME MIX 5U/UL 100U

UNSPSC: 41106300

Code: 88

Additional Details:
Additional Details: Weight: 0.03000kg



Disclaimers: This product is licensed under one U.S. Patent No. 5,436,149 or corresponding foreign patents owned by Takara Bio, Inc.

Product Code. 10460280

Quantity Price
1 £ 72.0 / 100 units
EU Stock 23
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Description and Specification

Specification

Description Long PCR Enzyme Mix
For Use With (Application) Long range PCR; PCR from GC-rich or difficult templates; Generation of template for TA cloning; RT-PCR.
Quantity 100U

The two enzymes synergistically generate long PCR products with greater yield and fidelity than Taq DNA Polymerase alone. The fidelity of PCR with this enzyme mix is three times higher than with Taq DNA Polymerase. The ratio of enzymes in the Long PCR Enzyme Mix is optimized for generation of very long amplicons: up to 47kb with viral DNA and up to 21kb with genomic DNA templates (see Fig. 1). The specially formulated Long PCR Buffer protects DNA from depurination and nicking during long thermal cycling. The products generated with the Long PCR Enzyme Mix are mostly 3'-dA tailed.Long PCR Enzyme Mix is also used for efficient amplification of GC-rich DNA regions (see Fig. 2).
  • Unique blend of two thermostable DNA polymerases
  • Long PCR products:—up to 47kb with viral DNA as template,—up to 21kb with genomic DNA as template
  • Ideal for GC-rich templates up to 85%
  • Fidelity is three-times higher than with Taq DNA polymerase (see Fig. 1)
  • High yield

Recommended for:

Long range PCR; PCR from GC-rich or difficult templates; Generation of template for TA cloning; RT-PCR.