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Thermo Scientific™ LguI (SapI)

The LguI (SapI) restriction enzyme recognizes GCTCTTC(1/4)^ sites and cuts best at 37°C in Tango buffer (Isoschizomers: BspQI, PciSI, SapI).

Brand:  Thermo Scientific™ ER1932

Code : 88

Additional Details : Weight : 0.00850kg

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Product Code. 10660661

  • £241.09 / 500 units

EU Stock 13
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5'..G C T C T T C (N)1▵...3'

3'..C G A G A A G (N)4▵...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • LguI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: may overlap —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: may overlap —effect not determined

Digestion of Agarose-embedded DNA:

  • Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours


Low salt, high glycerol (>5%) concentrations, pH >8.0 or a large excess of enzyme may result in star activity.



LguI (SapI)