Lambda DNA/EcoRI+HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Fermentas PureExtreme restriction enzyme(s), purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.
Lambda DNA/EcoRI+HindIII Marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively, it can be labeled radioactively or non-radioactively with Klenow Fragment or Klenow Fragment, exo- with the fill-in reactio
Sizing and approximate quantification of large DNA fragments
Supplied with loading dye for sample DNA
The cohesive ends (the 12 nt cos site of lambda DNA) of fragments indicated™ may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5 min. and then cooling on ice for 3 min.
The shortest fragments (oblique) are not visible in standard electrophoresis. Fragment lengths are predicted by computer analysis of respective DNA sequences.