Thermo Scientific™ HpyF3I (DdeI)

The HpyF3I (DdeI) restriction enzyme recognizes C^TNAG sites and cuts best at 37°C in Tango buffer (Isoschizomers: BstDEI, DdeI).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER1882

2500 UNIT RESTRICTION ENZYME, CONVENTIONAL, HPYF3I(DdeI), 10u/ul, cuts C¬TNAG at 37°C (supplied

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.00880kg



Product Code. 10889710

Quantity Price
1 £ 141.0 / 2500 units
EU Stock 4
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Description and Specification

Specification

Product Name HpyF3I (DdeI)
Concentration 10U/µL
Units 2,500U

5'..C▵T N A G...3'

3'..G A N T▵C...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • HpyF3I is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: never overlaps —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: may overlap —effect not determined

Compatible Ends:

  • BbvCI, Bpu10I, Bpu1102I, Eco81I