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Thermo Scientific™ HpyF3I (DdeI)

The HpyF3I (DdeI) restriction enzyme recognizes C^TNAG sites and cuts best at 37°C in Tango buffer (Isoschizomers: BstDEI, DdeI).

Brand:  Thermo Scientific™ ER1882

UNSPSC : 12352204

Code : 88

Additional Details : Weight : 0.00880kg

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Product Code. 10889710

  • £141.00 / 2500 units


EU Stock 2
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Specifications

Description

5'..C▵T N A G...3'

3'..G A N T▵C...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • HpyF3I is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: never overlaps —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: may overlap —effect not determined

Compatible Ends:

  • BbvCI, Bpu10I, Bpu1102I, Eco81I
Description & Specifications

Specifications

HpyF3I (DdeI)
10U/µL
2,500U