Thermo Scientific™ HpyF10VI (MwoI)

The HpyF10VI (MwoI) restriction enzyme recognizes GCNNNNN^NNGC sites and cuts best at 37°C in Tango buffer (Isoschizomers: BstMWI, MwoI).

Brand:  Thermo Scientific™ ER1731

Product Code. 10744681

Description

5'..G C N N N N N▵N N G C...3'

3'..C G N N▵N N N N N C G...5'

Conditions for 100% Activity:

• 1X Buffer Tango™: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
• Incubate at 37°C

Storage Buffer:

• HpyF10VI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

• Dam: never overlaps —no effect
• Dcm: never overlaps —no effect
• CpG: may overlap —cleavage impaired
• EcoKI: never overlaps —no effect
• EcoBI: never overlaps —no effect

Specifications

• Product Name: HpyF10VI (MwoI)
• Concentration: 10U/µL
• Units: 300U