Thermo Scientific™ HpyF10VI (MwoI)

The HpyF10VI (MwoI) restriction enzyme recognizes GCNNNNN^NNGC sites and cuts best at 37°C in Tango buffer (Isoschizomers: BstMWI, MwoI).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER1731

RESTRICTION ENZYME, CONVENTIONAL, HPYF10VI (MWOI),10u/ul, cuts GCNNNNN¬NNGC at 37°C (supplied with

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.00900kg



Product Code. 10744681

Quantity Price
1 £ 40.83 / 300 units
EU Stock 6
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Description and Specification

Specification

Product Name HpyF10VI (MwoI)
Concentration 10U/µL
Units 300U

5'..G C N N N N N▵N N G C...3'

3'..C G N N▵N N N N N C G...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • HpyF10VI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: may overlap —cleavage impaired
  • EcoKI: never overlaps —no effect
  • EcoBI: never overlaps —no effect