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Thermo Scientific™ Hpy8I (MjaIV)

The Hpy8I (MjaIV) restriction enzyme recognizes GTN^NAC sites and cuts best at 37°C in Tango buffer (Isoschizomers: Hpy166II).

Brand:  Thermo Scientific™ ER1572

UNSPSC : 12352204

Code : 88

Additional Details : Weight : 0.00900kg

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Product Code. 10480090

  • £233.00 / 1000 units


Estimated Shipment
13-06-2017
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Specifications

Description

5'..G T N▵N A C...3'

3'..C A N▵N T G...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • Hpy8I is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: may overlap —cleavage impaired
  • EcoKI: may overlap —effect not determined
  • EcoBI: may overlap —effect not determined
Description & Specifications

Specifications

Hpy8I (MjaIV)
10U/µL
1,000U