Thermo Scientific™ Hpy8I (MjaIV)

The Hpy8I (MjaIV) restriction enzyme recognizes GTN^NAC sites and cuts best at 37°C in Tango buffer (Isoschizomers: Hpy166II).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER1572

1000 UNIT RESTRICTION ENZYME, CONVENTIONAL, HPY8I(MjaIV), 10u/ul, cuts GTN¬NAC at 37°C (supplied

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.00900kg



Product Code. 10480090

Quantity Price
1 £ 233.0 / 1000 units
EU Stock 1
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Description and Specification

Specification

Product Name Hpy8I (MjaIV)
Concentration 10U/µL
Units 1,000U

5'..G T N▵N A C...3'

3'..C A N▵N T G...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • Hpy8I is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: may overlap —cleavage impaired
  • EcoKI: may overlap —effect not determined
  • EcoBI: may overlap —effect not determined