Thermo Scientific™ HphI

The HphI restriction enzyme recognizes GGTGA(8/7)^ sites and cuts best at 37°C in B buffer (Isoschizomers: AsuHPI).

Brand: Thermo Scientific™

Manufacturer Part Number: ER1101

300 UNIT RESTRICTION ENZYME, CONVENTIONAL, HPHI,10u/ul, cuts GGTGA(8/7)¬ at 37°C (supplied with

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg

Product Code. 10679900

Quantity Price
1 £17.69 / 300 units
EU Stock 22
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Description and Specification


Product Name HphI
Concentration 10U/µL
Units 300U

5'...G G T G A (N)8▵...3'

3'...C C A C T (N)7▵...5'

Conditions for 100% Activity:

  • 1X Buffer B: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2 and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • HphI is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: may overlap —blocked
  • Dcm: may overlap —no effect
  • CpG: may overlap —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: may overlap —blocked


Assayed using lambda DNA (dam) (#SD0021). HphI is blocked by overlappingdam methylation. To avoiddam methylation, use adam-, dcm strain such as GM2163 (#M0099). High glycerol (>5%) concentrations, pH>8.0 or a large excess of enzyme may result in star activity.