Thermo Scientific™ HinfI

The HinfI restriction enzyme recognizes G^ANTC sites and cuts best at 37°C in R buffer.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER0803

RESTRICTION ENZYME, CONVENTIONAL, HINFI, 50U/UL,HC, cuts G¬ANTC at 37°C (supplied with 1mL of 10X

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 10579120

Quantity Price
1 £ 92.5 / 10000 units
EU Stock 2
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Description and Specification

Specification

Product Name HinfI
Concentration 50U/µL
Units 10,000U

5'...G▵A N T C...3'

3'...C T N A▵G...5' Conditions for 100% Activity:

  • 1X Buffer R: 10mM Tris-HCl (pH8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • HinfI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: may overlap —cleavage impaired
  • EcoKI: never overlaps —no effect
  • EcoBI: may overlap —blocked

Compatible Ends:

  • G▴AWTC — PfeI