Thermo Scientific™ HinfI
The HinfI restriction enzyme recognizes G^ANTC sites and cuts best at 37°C in R buffer.
Brand: Thermo Scientific™ ER0803
Code : 88
Additional Details : Weight : 0.01000kg
5'...G▵A N T C...3'
3'...C T N A▵G...5' Conditions for 100% Activity:
- 1X Buffer R: 10mM Tris-HCl (pH8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
- Incubate at 37°C
- HinfI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol
- Dam: never overlaps —no effect
- Dcm: never overlaps —no effect
- CpG: may overlap —cleavage impaired
- EcoKI: never overlaps —no effect
- EcoBI: may overlap —blocked
- G▴AWTC — PfeI