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Thermo Scientific™ HincII (HindII)

The HincII (HindII) restriction enzyme recognizes GTY^RAC sites and cuts best at 37°C in Tango buffer (Isoschizomers: HindII).

Brand:  Thermo Scientific™ ER0492

UNSPSC : 12352204

Code : 88

Additional Details : Weight : 1.51950kg

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Product Code. 10808810

  • £107.00 / 2500 units


EU Stock 3
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Specifications

Description

5'...G T Y▵R A C...3'

3'...C A R▵Y T G...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™: 33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • HincII is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: may overlap —cleavage impaired
  • EcoKI: may overlap —blocked
  • EcoBI: may overlap —blocked
Description & Specifications

Specifications

HincII (HindII)
10U/µL
2,500U