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Thermo Scientific™ GsuI (BpmI)

The GsuI (BpmI) restriction enzyme recognizes CTGGAG(16/14)^ sites and cuts best at 30°C in B buffer (Isoschizomers: BpmI).

Brand:  Thermo Scientific™ ER0462

Code : 88

Additional Details : Weight : 1.51950kg

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Product Code. 10869360

  • £200.00 / 500 units


Estimated Shipment
10-01-2018
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Description

Description

5'...C T G G A G (N)16▵...3'

3'...G A C C T C (N)14▵...5'

Conditions for 100% Activity:

  • 1X Buffer B: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2 and 0.1mg/mL BSA
  • Incubate at 30°C

Storage Buffer:

  • GsuI is supplied in:10mM potassium phosphate (pH 7.5 at 25°C), 1mM EDTA, 1mM DTT, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: may overlap —blocked
  • CpG: never overlaps —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: never overlaps —no effect

Note:

  • Incubation at 37°C results in 70% activity
  • GsuI requires only Mg2 + for its activity, but is stimulated by S-adenosylmethionine
  • 0.01mM S-adenosylmethionine gives a 2-fold increase in activity
  • GsuI may remain associated with the cleaved DNA
  • This may cause DNA band shifting during electrophoresis
  • To avoid atypical DNA band patterns, use the 6X DNA Loading Dye and SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis
  • GsuI is blocked by overlappingdcm methylation
  • To avoiddcm methylation, use adam,dcm- strain such as GM2163 (#M0099)
Specifications

Specifications

GsuI (BpmI)
5U/μL
500U
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