More than 80% of the digested DNA fragments can be ligated and more than 90% can be recut at a 5ft.-termini concentration of 0.2μM after a 50-fold overdigestion (3U/μg DNA x 17 hours) with FspAI.
5'...R T G C▵G C A Y...3'
3'...Y A C G▵C G T R...5'
Conditions for 100% Activity:
Low salt, high glycerol (>5%) concentrations, pH >8.0 or a large excess of enzyme may result in star activity.