All FastDigest enzymes are 100% active in the universal FastDigest and FastDigest Green buffers and are able to digest DNA in 5-15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. As an added convenience, the FastDigest Green Buffer allows for direct loading of the reaction mixture on a gel. FastDigest enzymes can be used to digest plasmid, genomic and viral DNA as well as PCR products and do not show star activity even in prolonged incubations.
5'..C T G C A▵G...3'
3'..G▵A C G T C...5'
100% activity of all FastDigest enzymes in the new green universal digestion buffer
100% buffer compatibility with all downstream applications
Complete digestion in 5-15 minutes
Direct loading of reaction mixture on gels
Two versions of the universal FastDigest buffer are supplied with each enzyme: 10X FastDigest Buffer and 10X FastDigest Green Buffer. All FastDigest enzymes are 100% active in both buffers. Enzymes used in common downstream applications such as ligation, blunting and dephosphorylation also have 100% activity in both buffers.
Fast clone analysis; Fast preparation of DNA for cloning; Digestion of PCR products; Fast RFLP genotyping; Digestion of difficult-to-cleave DNA