Thermo Scientific™ FastDigest™ NcoI

The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: FD0574

100 UL RESTRICTION ENZYME, FASTDIGEST, NCOI, CUTSC¬CATGG, 1µL cleaves 1µg DNA in 5 minutes at 37°C

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01780kg



Product Code. 10560841

Quantity Price
1 £ 44.79 / 100 reactions
EU Stock 73
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Description and Specification

Specification

Product Name FastDigest NcoI
Components 10X FastDigest Buffer and 10X FastDigest Green Buffer
Quantity 100µL
For Use With (Application) Fast clone analysis; Fast preparation of DNA for cloning; Digestion of PCR products; Fast RFLP genotyping; Digestion of difficult-to-cleave DNA

All FastDigest enzymes are 100% active in the universal FastDigest and FastDigest Green buffers and are able to digest DNA in 5-15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. As an added convenience, the FastDigest Green Buffer allows for direct loading of the reaction mixture on a gel. FastDigest enzymes can be used to digest plasmid, genomic and viral DNA as well as PCR products and do not show star activity even in prolonged incubations.

5'...C▵C A T G G...3'

3'...G G T A C▵C...5'

  • 100% activity of all FastDigest enzymes in the new green universal digestion buffer
  • 100% buffer compatibility with all downstream applications
  • Complete digestion in 5-15 minutes
  • Direct loading of reaction mixture on gels

Includes:

Two versions of the universal FastDigest buffer are supplied with each enzyme: 10X FastDigest Buffer and 10X FastDigest Green Buffer. All FastDigest enzymes are 100% active in both buffers. Enzymes used in common downstream applications such as ligation, blunting and dephosphorylation also have 100% activity in both buffers.

Recommended for:

Fast clone analysis; Fast preparation of DNA for cloning; Digestion of PCR products; Fast RFLP genotyping; Digestion of difficult-to-cleave DNA