Thermo Scientific™ Eco91I (BstEII)

The Eco91I (BstEII) restriction enzyme recognizes G^GTNACC sites and cuts best at 37°C in O buffer (Isoschizomers: BstEII, BstPI, EcoO65I, PspEI).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER0392

5000 UNIT RESTRICTION ENZYME, CONVENTIONAL, ECO91I(BstEII),10u/uL, cuts G¬GTNACC at 37°C (supplied

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Product Code. 10859850

Quantity Price
1 £ 105.0 / 5000 units
EU Stock 12
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Description and Specification

Specification

Product Name Eco91I (BstEII)
Concentration 10U/µL
Quantity 5,000U

5'...G▵G T N A C C...3'

3'...C C A N T G▵G...5'

Conditions for 100% Activity:

  • 1X Buffer O: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • Eco91I is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with Eco91I, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps-no effect
  • Dcm: may overlap-no effect
  • CpG: may overlap-no effect
  • EcoKI: may overlap-effect not determined
  • EcoBI: may overlap-effect not determined

Digestion of Agarose-embedded DNA:

  • Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Compatible Ends:

  • G▵GTSACC-MaeIII, NmuCI;G▵GTWACC-MaeIII