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Thermo Scientific™ Eco81I (Bsu36I)

The Eco81I (Bsu36I) restriction enzyme recognizes CC^TNAGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: AxyI, Bse21I, Bsu36I).

Brand:  Thermo Scientific™ ER0372

Code : 88

Additional Details : Weight : 0.00900kg

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Product Code. 10849180

  • £217.74 / 2500 units

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5'...C C▵T N A G G...3'
  • 3'...G G A N T▵C C...5' Conditions for 100% Activity:
    • 1X Buffer Tango: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
    • Incubate at 37°C
    Storage Buffer:
    • Eco81I is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol
    Ligation and Recleavage:
    • After 10-fold overdigestion with Eco81I, more than 80% of the M13mp18 DNA fragments can be ligated in a reaction mixture containing 20-40U of T4 DNA Ligase/1μg of fragments and 10% PEG
    • More than 95% of these can be recut
    Methylation Effects:
    • Dam: never overlaps-no effect
    • Dcm: never overlaps-no effect
    • CpG: never overlaps-no effect
    • EcoKI: never overlaps-no effect
    • EcoBI: may overlap-effect not determined
    Digestion of Agarose-embedded DNA:
    • Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours
    Compatible Ends:
    • BbvCI, Bpu10I, Bpu1102I, HpyF3I
  • Specifications


    Eco81I (Bsu36I)