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Thermo Scientific™ Eco47III (AfeI)

The Eco47III (AfeI) restriction enzyme recognizes AGC^GCT sites and cuts best at 37°C in O buffer (Isoschizomers: AfeI, Aor51HI).

Brand:  Thermo Scientific™ ER0322

Code : 88

Additional Details : Weight : 0.01000kg

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Product Code. 10690071

  • £314.39 / 1000 units

EU Stock 7
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5'...A G C▵G C T...3'

3'...T C G▵C G A...5'

Conditions for 100% Activity:

  • 1X Buffer O:50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • Eco47III is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with Eco47III, more than 80% of pBR322 DNA fragments can be ligated and more than 90% of these can be recut

Methylation Effects:

  • Dam: never overlaps-no effect
  • Dcm: never overlaps-no effect
  • CpG: completely overlaps-blocked
  • EcoKI: never overlaps-no effect
  • EcoBI: may overlap-effect not determined

Digestion of Agarose-embedded DNA:

  • Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours


Eco47III (AfeI)