Thermo Scientific™ Eco31I (BsaI)

The Eco31I (BsaI) restriction enzyme recognizes GGTCTC(1/5)^ sites and cuts best at 37°C in G buffer (Isoschizomers: BsaI, Bso31I, BspTNI).

Brand: Thermo Scientific™

Manufacturer Part Number: ER0291

ECO31I (BSAI) 10U/UL 1000U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg

Product Code. 10548920

Quantity Price
1 £53.00 / 1000 units
EU Stock 17
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Description and Specification


Product Name Eco31I (BsaI)
Concentration 10U/µL
Incubator Temperature 37°C
Quantity 1000U

5'...G G T C T C (N)1▵...3'

3'...C C A G A G (N)5▵...5'

Conditions for 100% Activity:

  • 1X Buffer G:10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 50mM NaCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • Eco31I is supplied in:10mM Tris-HCl (pH 7.5 at 25°C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with Eco31I, more than 95% of DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps-no effect
  • Dcm: may overlap-cleavage impaired
  • CpG: may overlap-cleavage impaired
  • EcoKI: never overlaps-no effect
  • EcoBI: may overlap-effect not determined

Digestion of Agarose-embedded DNA:

  • Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours


Assayed using lambda DNA (dcm-) (#SD0021), as one of the two Eco31I recognition sites in lambda DNA is difficult to cleave. Eco31I cleavage is impaired by overlappingdcm methylation. To avoiddcm methylation, use adam,dcm- strain such as GM2163 (#M0099). Low salt, high glycerol (>5%) concentrations, pH >8.0 or a large excess of enzyme may result in star activity. Eco31I cleaves downstream of its recognition site and can generate any desired 4 base 5'-overhangs. This feature is useful for direct PCR product cloning.