Thermo Scientific™ DecaLabel™ Biotin DNA Labeling Kit
Efficiently synthesize biotin-labeled DNA probes with the Thermo Scientific™ Biotin DecaLabel DNA Labeling Kit.
Brand: Thermo Scientific™ K0652
Code : 88
Additional Details : Weight : 1.58760kg
DescriptionThe primary improvement over traditional random-primed method involves the use of random decamers instead of hexamers to ensure more efficient annealing with DNA at 37°C. Klenow Fragment, exo- is also included in the kit; this genetically engineered enzyme has no detectable exonuclease activity. Therefore, the enzyme does not degrade the labeled probe during reaction, which results in a high labeling yield even with low amounts of template. As a result, DNA fragments of any length can be uniformly labeled. Biotin-labeled DNA is detected with the Biotin Chromogenic Detection Kit or with conventional biotin-avidin or biotin-streptavidin detection systems.
- Non-radioactive labeling of DNA
- Efficient priming of labeling reactions with random decamers
- High yields with Klenow Fragment, exo-: no degradation of a labeled probe during reaction
Generation of biotin-labeled DNA probes for a variety of non-radioactive hybridization experiments, including Southern and Northern blots, colony/plaque hybridizations, dot/slot blots and in situ hybridizations
|Generation of biotin-labeled DNA probes for a variety of non-radioactive hybridization experiments, including Southern and Northern blots, colony/plaque hybridizations, dot/slot blots and in situ hybridizations|
|Klenow Fragment, exo-;Decanucleotides in Reaction Buffer;Biotin Labeling Mix;Control Template;Biotin-labeled DNA;Water, nuclease-free;Detailed Protocol|
|Biotin DecaLabel™ DNA Labeling Kit|