Thermo Scientific™ DecaLabel™ Biotin DNA Labeling Kit

Efficiently synthesize biotin-labeled DNA probes with the Thermo Scientificâ„¢ Biotin DecaLabel DNA Labeling Kit.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: K0652

30 RXN MOLECULAR LABELING, BIOTIN DECALABEL(TM)DNA labeling kit, includes Klenow fragment,exo-,

UNSPSC: 41105326

Code: 88

Additional Details:
Additional Details: Weight: 1.58760kg



Product Code. 10890231

Quantity Price
1 £ 215.0 / 30 reactions
EU Stock 2
Add to basket


Description and Specification

Specification

For Use With (Application) Generation of biotin-labeled DNA probes for a variety of non-radioactive hybridization experiments, including Southern and Northern blots, colony/plaque hybridizations, dot/slot blots and in situ hybridizations
Includes Klenow Fragment, exo-;Decanucleotides in Reaction Buffer;Biotin Labeling Mix;Control Template;Biotin-labeled DNA;Water, nuclease-free;Detailed Protocol
No. of Reactions 30
Product Type Biotin DecaLabelâ„¢ DNA Labeling Kit

The primary improvement over traditional random-primed method involves the use of random decamers instead of hexamers to ensure more efficient annealing with DNA at 37°C. Klenow Fragment, exo- is also included in the kit; this genetically engineered enzyme has no detectable exonuclease activity. Therefore, the enzyme does not degrade the labeled probe during reaction, which results in a high labeling yield even with low amounts of template. As a result, DNA fragments of any length can be uniformly labeled. Biotin-labeled DNA is detected with the Biotin Chromogenic Detection Kit or with conventional biotin-avidin or biotin-streptavidin detection systems.
  • Non-radioactive labeling of DNA
  • Efficient priming of labeling reactions with random decamers
  • High yields with Klenow Fragment, exo-: no degradation of a labeled probe during reaction

Recommended for:

Generation of biotin-labeled DNA probes for a variety of non-radioactive hybridization experiments, including Southern and Northern blots, colony/plaque hybridizations, dot/slot blots and in situ hybridizations