Thermo Scientific™ Cfr9I (XmaI)

The Cfr9I (XmaI) restriction enzyme recognizes C^CCGGG sites and cuts best at 37°C in its own unique buffer (Isoschizomers: TspMI, XmaCI, XmaI).

Brand: Thermo Scientific™

Manufacturer Part Number: ER0171

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg

Product Code. 10598560

Quantity Price
1 £ 24.95 / 300 units
EU Stock 12
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Description and Specification


Concentration 10U/µL
Incubator Temperature 37°C
Product Name Cfr9I (XmaI)
Quantity 300U

5'...C▵C C G G G...3'

3'...G G G C C▵C...5'

Conditions for 100% Activity:

  • 1X Buffer Cfr9I:10mM Tris-HCl (pH 7.2 at 37°C), 5mM MgCl2, 200mM sodium glutamate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • Cfr9I is supplied in:10mM Tris-HCl (pH 7.5 at 25°C), 250mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with Cfr9I, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps-no effect
  • Dcm: never overlaps-no effect
  • CpG: completely overlaps-cleavage impaired
  • EcoKI: never overlaps-no effect
  • EcoBI: never overlaps-no effect

Digestion of Agarose-embedded DNA:

  • Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours


To achieve complete digestion of substrate with Cfr9I, the concentration of DNA should be no less than 50μg/mL in the reaction buffer.