Thermo Scientific™ BstXI

Cut at CCANNNNN^NTGG sites with BstXI restriction enzyme, which performs best at 55°C in O buffer.

Brand: Thermo Scientific™

Manufacturer Part Number: ER1022

BSTXI 10U/UL 2500U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg

Product Code. 10599120

Quantity Price
1 £98.75 / 2500 units
EU Stock 8
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Description and Specification


Product Name BstXI
Concentration 10U/µL
Incubator Temperature 55°C
Quantity 2,500U

5'...C C A N N N N N▵N T G G...3'

3'...G G T N▵N N N N N A C C...5'

Conditions for 100% Activity:

  • b1X Buffer O: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl and 0.1mg/mL BSA
  • Incubate at 55°C

Storage Buffer:

  • BstXI is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with BstXI, approximately 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: may overlap — cleavage impaired
  • CpG: never overlaps — no effect
  • EcoKI: never overlaps — no effect
  • EcoBI: never overlaps — no effect

Digestion of Agarose-embedded DNA:

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours



Incubation at 37°C results in 50% activity. Greater than 15-fold overdigestion with BstXI may result in star activity. BstXI cleavage is impaired by overlappingdcm methylation. To avoiddcm methylation, use adam-, dcm strain such as GM2163 (#M0099).