Thermo Scientific™ BspLI (NlaIV)

Cut at GGN^NCC sites with BspLI (NlaIV) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BmiI, NlaIV, PspN4I).

Brand: Thermo Scientific™

Manufacturer Part Number: ER1151


UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.00900kg

Product Code. 10898990

Quantity Price
1 £41.61 / 200 units
EU Stock 9
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Description and Specification


Product Name BspLI (NlaIV)
Concentration 10U/µL
Incubator Temperature 37°C
Quantity 200U

5'..G G N▵N C C...3'

3'..C C N▵N G G...5'

Conditions for 100% Activity:

  • 1X Buffer Tango: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • BspLI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with BspLI, approximately 90% of the DNA fragments can be ligated and more than 95% of these can be recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: may overlap — cleavage impaired
  • CpG: may overlap — cleavage impaired
  • EcoKI: never overlaps — no effect
  • EcoBI: never overlaps — no effect


BspLI cleavage is impaired by overlappingdcm methylation. To avoiddcm methylation, use adam-, dcm strain such as GM2163 (#M0099).