Thermo Scientific™ Bsp143I (Sau3AI)

Cut at ^GATC sites with Bsp143I (Sau3AI), which performs best at 37°C in its own unique buffer (Isoschizomers: BfuCI, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, NdeII, Sau3AIm).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER0781

BSP143I (SAU3AI) 10U/UL 300U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Product Code. 10631811

Quantity Price
1 £ 19.58 / 300 units
EU Stock 11
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Description and Specification

Specification

Product Name Bsp143I (Sau3AI)
Concentration 10U/µL
Incubator Temperature 37°C
Quantity 300U

5'...▵G A T C ...3'

3'... C T A G▵...5'

Conditions for 100% Activity:

  • 1X Buffer Bsp143I: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate, 0.02% Triton X-100 and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • Bsp143I is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with Bsp143I more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: completely overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: may overlap — blocked
  • EcoKI: never overlaps — no effect
  • EcoBI: may overlap — no effect

Note:

DpnI, Bsp143I and MboI all recognize the same sequence but have different methylation sensitivities and cleavage sites. Compatible Ends: BamHI, BclI, BglII, PsuI.