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Thermo Scientific™ Bsh1236I (BstUI)

Cut at CG^CG sites with Bsh1236I (BstUI) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: AccII, BspFNI, BstFNI, BstUI, MvnI).

Brand:  Thermo Scientific™ ER0921

UNSPSC : 12352204

Code : 88

Additional Details : Weight : 1.51950kg

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Product Code. 10649310

  • £18.64 / 500 units

EU Stock 18
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5'...C G▵C G...3'

3'...G C▵G C...5'

Conditions for 100% Activity:

  • 1X Buffer R: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • Bsh1236I is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with Bsh1236I, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: completely overlaps — blocked
  • EcoKI: never overlaps — no effect
  • EcoBI: never overlaps — no effect


Bsh1236I (BstUI)