Thermo Scientific™ Bsh1236I (BstUI)

Cut at CG^CG sites with Bsh1236I (BstUI) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: AccII, BspFNI, BstFNI, BstUI, MvnI).

Brand: Thermo Scientific™

Manufacturer Part Number: ER0921

BSH1236I (BSTUI) 10U/UL 500U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg

Product Code. 10649310

Quantity Price
1 £22.89 / 500 units
EU Stock 28
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Description and Specification


Product Name Bsh1236I (BstUI)
Concentration 10U/µL
Incubator Temperature 37°C
Quantity 500U

5'...C G▵C G...3'

3'...G C▵G C...5'

Conditions for 100% Activity:

  • 1X Buffer R: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • Bsh1236I is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with Bsh1236I, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: completely overlaps — blocked
  • EcoKI: never overlaps — no effect
  • EcoBI: never overlaps — no effect