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Thermo Scientific™ BseGI (BtsCI) (10 U/μL)

Cut at GGATG(2/0)^ sites with BseGI (BtsCI) restriction enzyme, which performs best at 55°C in Tango buffer (Isoschizomers: BstF5I, BtsCI, FokI).

Brand:  Thermo Scientific™ ER0872

115.33 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : 88

Additional Details : Weight : 0.00900kg

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Product Code. 10490080

  • £173.00 / 2500 units
Estimated Shipment: 15-04-2024
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Description

Description

Conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'...G G A T G N N▵...3'

3'...C C T A C▵N N ...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™: 33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 55°C

Storage Buffer:

  • BseGI is supplied in:10mM Tris-HCl (pH7.4 at 25°C), 100mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with BseGI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: may overlap — no effect
  • CpG: may overlap — no effect
  • EcoKI: never overlaps — no effect
  • EcoBI: may overlap — effect not determined

Note:

Incubation at 37°C results in 25% activity.

Specifications

Specifications

10 U/μL
BseGI (BtsCI)
Yes
Yes
Restriction Enzyme
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
10X Buffer Tango
55°C
2500 U
Scarless Cloning
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For Research Use Only. Not for use in diagnostic procedures.