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Thermo Scientific™ BseGI (BtsCI)

Cut at GGATG(2/0)^ sites with BseGI (BtsCI) restriction enzyme, which performs best at 55°C in Tango buffer (Isoschizomers: BstF5I, BtsCI, FokI).

Brand:  Thermo Scientific™ ER0872

UNSPSC : 12352204

Code : 88

Additional Details : Weight : 0.00900kg

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Product Code. 10490080

  • £124.00 / 2500 units


Estimated Shipment
08-09-2017
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Description

Description

5'...G G A T G N N▵...3'

3'...C C T A C▵N N ...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™: 33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 55°C

Storage Buffer:

  • BseGI is supplied in:10mM Tris-HCl (pH7.4 at 25°C), 100mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with BseGI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: may overlap — no effect
  • CpG: may overlap — no effect
  • EcoKI: never overlaps — no effect
  • EcoBI: may overlap — effect not determined

Note:

Incubation at 37°C results in 25% activity.

Specifications

Specifications

BseGI (BtsCI)
10U/µL
55°C
2,500U