Thermo Scientific™ BplI

Cut at ^(8/13)GAG(N)5CTC(13/8)^ sites with BplI restriction enzyme, which performs best at 37°C in Tango(+SAM) buffer.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER1311

BPLI 5U/UL 100U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 10560851

Quantity Price
1 £ 43.57 / 100 units
EU Stock 2
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Description and Specification

Specification

Product Name BplI
Concentration 5U/µL
Incubator Temperature 37°C
Quantity 100U

5'...▵ 8(N) G A G (N)5 C T C (N)13▵...3'

3'...▵13(N) C T C (N)5 G A G (N)8 ▵...5'

Conditions for 100% Activity:

  • [1X Buffer Tango™] + SAM:[33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA] + 0.05mM S-adenosylmethionine
  • Incubate at 37°C

Storage Buffer:

  • BplI is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 10-fold overdigestion with BplI, more than 70% of the DNA fragments can be ligated, but none of these can be recut due to the methylation of the recognition sequence by this enzyme

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: may overlap — no effect
  • EcoKI: never overlaps — no effect
  • EcoBI: never overlaps — no effect

Digestion of Agarose-embedded DNA:

  • Minimum 10units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Note:

BplI requires only Mg2 + for its activity, but is stimulated by S-adenosylmethionine. 0.05mM S-adenosylmethionine gives more than a 100-fold increase in BplI activity. Still, complete cleavage of some substrates with BplI is difficult to achieve. BplI concentration is determined by the maximum cleavage level achieved when no change in the fragmentation pattern is observed with further enzyme increase.