5'...G A A G A C (N)2▵...3'
3'...C T T C T G (N)6▵...5'
Conditions for 100% Activity:
Ligation and Recleavage:
Digestion of Agarose-embedded DNA:
BpiI cleaves downstream of its recognition site and can generate any desired 4 base 5ft.-overhangs. This feature is useful for direct PCR product cloning.