missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ BpiI (BbsI) (10 U/μL)

Cut GAAGAC(2/6)^ sites with BpiI (BbsI) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: BbsI, BpuAI, BstV2I).

Brand:  Thermo Scientific™ ER1012

84.67 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : 88

Additional Details : Weight : 1.51950kg

 View more versions of this product

Product Code. 10609330

  • £127.00 / 1000 units
In Stock
to see stock.

Please to purchase this item. Need a web account? Register with us today!


Description

Description

Conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'...G A A G A C (N)2▵...3'

3'...C T T C T G (N)6▵...5'

Conditions for 100% Activity:

  • 1X Buffer G
  • 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 50mM NaCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • BpiI is supplied in:
  • 10mM Tris-HCl (pH7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with BpiI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: may overlap — no effect
  • EcoKI: never overlaps — no effect
  • EcoBI: may overlap — effect not determined

Digestion of Agarose-embedded DNA:

  • Minimum 5 units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Note:

BpiI cleaves downstream of its recognition site and can generate any desired 4 base 5ft.-overhangs. This feature is useful for direct PCR product cloning.

Specifications

Specifications

10 U/μL
BpiI (BbsI)
Yes
Yes
Restriction Enzyme
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
10X Buffer G
37°C
1000 U
Scarless Cloning
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.