Thermo Scientific™ BpiI (BbsI)

Cut GAAGAC(2/6)^ sites with BpiI (BbsI) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: BbsI, BpuAI, BstV2I).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER1012

RESTRICTION ENZYME, CONVENTIONAL, BPII (BBSI),10u/ul, cuts GAAGAC(2/6)¬ at 37°C (supplied with

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Product Code. 10609330

Quantity Price
1 £ 90.25 / 1000 units
EU Stock 45
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Description and Specification

Specification

Product Name BpiI (BbsI)
Concentration 10U/µL
Components 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 50mM NaCl and 0.1mg/mL BSA
Incubator Temperature 37°C
Quantity 1000U

5'...G A A G A C (N)2▵...3'

3'...C T T C T G (N)6▵...5'

Conditions for 100% Activity:

  • 1X Buffer G
  • 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 50mM NaCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • BpiI is supplied in:
  • 10mM Tris-HCl (pH7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with BpiI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: may overlap — no effect
  • EcoKI: never overlaps — no effect
  • EcoBI: may overlap — effect not determined

Digestion of Agarose-embedded DNA:

  • Minimum 5 units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Note:

BpiI cleaves downstream of its recognition site and can generate any desired 4 base 5ft.-overhangs. This feature is useful for direct PCR product cloning.