Thermo Scientific™ 5-Fluoroorotic Acid

Detect expression of the URA3 gene, which encodes orotine-5'-monophosphate (OMP) dicarboxylase with Thermo Scientificâ„¢ 5-Fluoroorotic Acid, used in yeast molecular genetics.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: R0811

1GR 5-FLUOROOROTIC ACID, >98% PURITY BY HPLCpowder 1g Store at -20°C

UNSPSC: 41116134

Code: 88

Additional Details:
Additional Details: Weight: 0.02000kg



Product Code. 10609920

Quantity Price
1 £ 65.5 / 1g
EU Stock 5
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Description and Specification

Specification

For Use With (Application) Detection of URA3 gene expression in yeast; Construction of yeast 2-hybrid libraries (1); Other areas of yeast genetics including gene replacement, analysis of transposable genetic elements and identification of gene mu
Product Type 5-Fluoroorotic Acid
Quantity 1g

Yeast with an active URA3 gene (Ura+) converts 5-FOA to fluorodeoxyuridine, which is toxic to cells (see the scheme below). Yeast strains carrying a mutation in the URA3 gene grow in the presence of 5-FOA if the media is supplemented with uracil. 5-FOA is frequently used in experiments with Saccharomyces cerevisiae (URA3), Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3) and E.coli (pyrF).

Recommended for:

Detection of URA3 gene expression in yeast; Construction of yeast 2-hybrid libraries (1); Other areas of yeast genetics including gene replacement, analysis of transposable genetic elements and identification of gene mutations.

Note:

Low solubility in water. Solubility in dimethylsulfoxide is greater than 150mg/mL. Preparation of a 100mg/mL solution in dime-thylsulfoxide is recommended.