FceR1 alpha Monoclonal Antibody (MAR-1), eFluor™ 450, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Product Code	Brand	Quantity
15599776	Affymetrix eBioscience 48-5898-82	100 μg
15589776	Affymetrix eBioscience 48-5898-80	25 μg

Description

Description: The MAR-1 monoclonal antibody reacts with the Fc epsilon Receptor I alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI alpha is expressed on mast and basophil cells and is up-regulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions. Applications Reported: This MAR-1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This MAR-1 antibody has been tested by flow cytometric analysis of MC/9 cell line (a mouse mast cell line). This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 μm post-manufacturing filtered.

FceR1 alpha Monoclonal antibody specifically detects FceR1 alpha in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: FceR1 alpha
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P20489
• Isotype: IgG
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: MAR-1
• Applications: Flow Cytometry
• Conjugate: eFluor 450
• Host Species: Armenian Hamster
• Target Species: Mouse
• Gene ID (Entrez): 14125
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!

For Research Use Only.