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FastAP Thermosensitive Alkaline Phosphatase

Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .

Brand:  Thermo Scientific EF0654

UNSPSC : 12352204

Code : 88

Additional Details : Weight : 0.01200kg

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Product Code. 10819230

  • £27.93 / 300 units


EU Stock 76
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Disclaimers:

The purchase of this product allows the purchaser to use it for preparing amplified DNA fragments under a license from GE Healthcare of U.S. Patent Nos. 5,741,676 and 5,756,285 and other foreign patents.


Description

Description

Highlights

  • Recombinant enzyme
  • Fast dephosphorylation – 10 minutes at 37°C
  • Fast and complete inactivation – 5 minutes at 75°C
  • Simultaneous digestion and dephosphorylation of vector DNA
  • 100% active in restriction enzyme and PCR buffers
  • One protocol for all types of DNA ends:
    • 5’-overhangs
    • 3’-overhangs
    • blunt-ends
    • single nucleotides
  • PCR clean-up in conjunction with Exo I
  • Protein dephosphorylation

Applications

  • Dephosphorylation of cloning vector DNA to prevent recircularization during ligation
  • Simultaneous digestion and dephosphorylation of vector DNA
  • PCR product clean-up: nucleotide degradation prior to sequencing of PCR product
  • Dephosphorylation of nucleic acid 5'-termini prior to labeling with T4 Polynucleotide Kinase
  • Other applications where dephosphorylation of DNA and RNA substrates is necessary
  • Protein dephosphorylation

Note

  • Binding of FastAP Thermosensitive Alkaline Phosphatase to DNA may result in a band shift in agarose gels. To avoid this, incubate samples with 6X DNA Loading Dye & SDS Solution at 65°C for 10 minutes, and chill on ice prior to electrophoresis.
  • FastAP Thermosensitive Alkaline Phosphatase is active in all restriction enzyme buffers and may be added directly to digested DNA. Heat inactivation of the restriction enzyme before dephosphorylation reaction is not necessary.
Specifications

Specifications

300 reactions
metal chelators
E.coli cells with a cloned bacterial AP gene
300U