Invitrogen™ FABP-3 Human ELISA Kit

Sandwich ELISA Kit

Product Code	Brand	Quantity
15974204	Invitrogen™ BMS2263	96 Tests
15984204	Invitrogen™ BMS2263TEN	96 x 10 Tests

Description

The Human FABP-3 ELISA quantitates Hu FABP-3 in human serum, plasma or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FABP-3.

Principle of the method

The Human FABP-3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The intracellular fatty acid-binding proteins belong to a multigene family. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism, and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. The heart-type fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is a candidate tumor suppressor gene for human breast cancer.

Specifications

• Accession Number: P05413
• Assay Sensitivity: 26.88 pg/ml
• Product Type: ELISA Kit
• Target Species: Human
• Gene ID (Entrez): 2170
• Interassay CV: 6.2%
• Kit Contents: Pre-coated 96-well Plate, Standard (2 vials), Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromagen, Stop Solution, Adhesive Plate Covers
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.

• Assay Range: 156.3-5000 pg/ml
• Conjugate: Biotin
• Sample Type: Cell Lysates, Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: FABP3
• Intraassay CV: 3.9%
• Label or Dye: HRP
• Sample Volume: Serum, 50 μL; Plasma, 50 μL; Cell Lysate,50 μL; Supernatant, 50 μL
• Target: Human
• Total Assay Time: 4 hr.