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F4/80, Functional Grade, clone: BM8, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-4801-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15268627

  • £187.40 / 500µg


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Disclaimers:

For Research Use Only.


Description

Description

Description: The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximately 125kDa transmembrane protein. The F4/80 antigen is expressed by a majority of mature macrophages and is the best marker for this population of cells. However, other cell types such as Langerhans cells and liver Kupffer cells have been reported to express this antigen. Expression of F4/80 commences during early myeloid development and is upregulated on all BM cells stimulated in vitro with M-CSF. It has been shown that some cytokines downregulate the expression of F4/80 resulting in lack of F4/80 antigen on a subpopulation of macrophages, especially in the lymphoid microenvironment in vivo.Applications Reported: BM8 has been reported for use in flow cytometric analysis.Applications Tested: The BM8 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

F4/80 Antigen
BM8
Unconjugated
Purified
Rat
500μg
Store at 2-8°C.
Monoclonal
Flow Cytometry
1mg/mL
aqueous buffer, no sodium azide
Pan Macrophage Marker
IgG2a
RUO
Primary
Murine
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