F4/80, clone: BM8, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-4801-85
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximately 160kDa surface receptor. It belongs to the EGF-TM7 family of proteins. As such it contains seven EGF-like domains on its extracellular N-terminus, seven transmembrane spanning sequences, and an intracellular C-terminal domain showing homology to other TM7 superfamily members. The F4/80 antigen is expressed by a majority of mature macrophages and is one of the best markers for this population of cells. However, other cell types, such as peritoneal eosinophils, Langerhans cells, and some other dendritic cell subtypes, have been reported to express this antigen as well. Expression of F4/80 commences during early myeloid development in vivo and can be upregulated on BM cells stimulated in vitro with M-CSF. Some populations of macrophages, especially in the lymphoid microenvironment, may be devoid of F4/80.Applications Reported: The BM8 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of paraffin embedded tissue sections. Immunoblotting must be performed under non-reducing conditions. For immunohistochemical staining of paraffin sections, proteinase treatment is needed. It has been reported that reduction with 2-mercaptoenthanol destroys the BM8 antigen.Applications Tested: The BM8 antibody has been tested by flow cytometric analysis of mouse spleen or bone marrow cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The BM8 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using pronase treatment and can be used at less than or equal to 10 μg/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|DD7A5-7, EGF-TM7, F4/80, Gpf480, Ly71, TM7LN3|
|Flow CytometryþImmunohistochemistryþImmunohistochemistry (Frozen)|
|Store at 2-8°C.|
For Research Use Only.