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F4/80, APC-eFluor 780, clone: BM8, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 47-4801-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15311660

  • £211.39 / 100µg

EU Stock 9
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Description: The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximately 160kDa surface receptor. It belongs to the EGF-TM7 family of proteins. As such it contains seven EGF-like domains on its extracellular N-terminus, seven transmembrane spanning sequences, and an intracellular C-terminal domain showing homology to other TM7 superfamily members. The F4/80 antigen is expressed by a majority of mature macrophages and is one of the best markers for this population of cells. However, other cell types, such as peritoneal eosinophils, Langerhans cells, and some other dendritic cell subtypes, have been reported to express this antigen as well. Expression of F4/80 commences during early myeloid development in vivo and can be upregulated on BM cells stimulated in vitro with M-CSF. Some populations of macrophages, especially in the lymphoid microenvironment, may be devoid of F4/80.Applications Reported: This BM8 antibody has been reported for use in flow cytometric analysis.Applications Tested: This BM8 antibody has been tested by flow cytometric analysis of mouse resident peritoneal exudate cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.APC-eFluor® emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.


F4/80 Antigen
PBS with 0.09% sodium azide; pH 7.2
DD7A5-7, EGF-TM7, F4/80, Gpf480, Ly71, TM7LN3
Affinity chromatography
Flow Cytometry
APC-eFluor 780
IgG2a, kappa
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.

For Research Use Only.