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F4/80 Monoclonal Antibody (BM8), APC, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

£78.00 - £213.00

Specifications

Antigen F4/80
Clone BM8
Concentration 0.2 mg/mL
Applications Flow Cytometry
Classification Monoclonal
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15350730
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Affymetrix eBioscience
17-4801-80
25 μg
£78.00
25µg
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Estimated Shipment: 19-04-2024
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15360730
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Affymetrix eBioscience
17-4801-82
100 μg
£213.00
100µg
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Description

Description

Description: The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximately 160 kDa surface receptor. It belongs to the EGF-TM7 family of proteins. As such it contains seven EGF-like domains on its extracellular N-terminus, seven transmembrane spanning sequences, and an intracellular C-terminal domain showing homology to other TM7 superfamily members. The F4/80 antigen is expressed by a majority of mature macrophages and is one of the best markers for this population of cells. However, other cell types, such as peritoneal eosinophils, Langerhans cells, and some other dendritic cell subtypes, have been reported to express this antigen as well. Expression of F4/80 commences during early myeloid development in vivo and can be upregulated on BM cells stimulated in vitro with M-CSF. Some populations of macrophages, especially in the lymphoid microenvironment, may be devoid of F4/80. Applications Reported: BM8 has been reported for use in flow cytometric analysis. Applications Tested: The BM8 antibody has been tested by flow cytometric analysis of mouse resident peritoneal exudate cells. This can be used at less than or equal to 2 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

F4/80 Monoclonal antibody specifically detects F4/80 in Mouse samples. It is validated for Flow Cytometry.
Specifications

Specifications

F4/80
0.2 mg/mL
Monoclonal
Liquid
RUO
PBS with 0.09% sodium azide; pH 7.2
ADGRE1; Adhesion G protein-coupled receptor E1; Adhesion G protein-coupled receptor E2; cell surface glycoprotein EMR1; cell surface glycoprotein F4/80; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like 1; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly71; lymphocyte antigen 71; TM7LN3
ADGRE1
Primary
4° C, store in dark, DO NOT FREEZE!
ADGRE1
BM8
Flow Cytometry
APC
Rat
Mouse
Q61549
13733
IgG2a κ
Affinity chromatography
Antibody
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