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F4/80 Rat anti-Mouse, Alexa Fluor® 700, Clone: BM8, eBioscience™

Rat Monoclonal Antibody

£80.25 - £205.00

Specifications

Antigen F4/80
Clone BM8
Target Species Mouse
Conjugate Alexa Fluor™ 700
Isotype IgG2a, kappa
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15590357
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Affymetrix eBioscience
56-4801-80
25 μg
£80.25
25µg
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Estimated Shipment
03-03-2021
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15500367
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Affymetrix eBioscience
56-4801-82
100 μg
£205.00
100µg
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Estimated Shipment
03-03-2021
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Description

Description

Description: The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximately 160 kDa surface receptor. It belongs to the EGF-TM7 family of proteins. As such it contains seven EGF-like domains on its extracellular N-terminus, seven transmembrane spanning sequences, and an intracellular C-terminal domain showing homology to other TM7 superfamily members. The F4/80 antigen is expressed by a majority of mature macrophages and is one of the best markers for this population of cells. However, other cell types, such as peritoneal eosinophils, Langerhans cells, and some other dendritic cell subtypes, have been reported to express this antigen as well. Expression of F4/80 commences during early myeloid development in vivo and can be upregulated on BM cells stimulated in vitro with M-CSF. Some populations of macrophages, especially in the lymphoid microenvironment, may be devoid of F4/80. Applications Reported: This BM8 antibody has been reported for use in flow cytometric analysis. Applications Tested: This BM8 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome. Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered. F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.
Specifications

Specifications

F4/80
Mouse
IgG2a, kappa
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
BM8
Alexa Fluor™ 700
Flow Cytometry
Primary
Monoclonal
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
RUO
SDS

For Research Use Only.