F4/80 Rat anti-Mouse, Alexa Fluor® 700, Clone: BM8, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 56-4801-80

Product Code. 15590357

Description

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome. Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered. F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

Specifications

• Antigen: F4/80
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q61549
• Gene Alias: DD7A5-7; EGF-TM7; F4/80; Gpf480; Ly71; TM7LN3; F480
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Mouse

• Applications: Flow Cytometry
• Clone: BM8
• Conjugate: Alexa Fluor™ 700
• Gene: Adgre1
• Format: Liquid
• Gene Symbols: Adgre1
• Isotype: IgG2a, kappa
• Quantity: 25 μg
• Storage Requirements: 4° C, store in dark, DO NOT FREEZE!
• Primary or Secondary: Primary
• Gene ID (Entrez): 13733

For Research Use Only.