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F4/80, Alexa Fluor 700, clone: BM8, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 56-4801-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15500367

  • £195.95 / 100µg


Estimated Shipment date
from Supplier 30-07-2018
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Description

Description

Description: The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximately 160kDa surface receptor. It belongs to the EGF-TM7 family of proteins. As such it contains seven EGF-like domains on its extracellular N-terminus, seven transmembrane spanning sequences, and an intracellular C-terminal domain showing homology to other TM7 superfamily members. The F4/80 antigen is expressed by a majority of mature macrophages and is one of the best markers for this population of cells. However, other cell types, such as peritoneal eosinophils, Langerhans cells, and some other dendritic cell subtypes, have been reported to express this antigen as well. Expression of F4/80 commences during early myeloid development in vivo and can be upregulated on BM cells stimulated in vitro with M-CSF. Some populations of macrophages, especially in the lymphoid microenvironment, may be devoid of F4/80.Applications Reported: This BM8 antibody has been reported for use in flow cytometric analysis.Applications Tested: This BM8 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

F4/80 Antigen
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q61549
DD7A5-7, EGF-TM7, F4/80, Gpf480, Ly71, TM7LN3
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
BM8
Alexa Fluor 700
Adgre1
Conjugated
Adgre1
IgG2a, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
13733
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