Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin and Labeling Kits

Biotinylate protein amines (e.g., lysines) with this very popular mid-length (22.4A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 21335

100 MG SULFO-NHS-LC-BIOTIN, EZ-LINK 100MG STOREin Freezer at -20C

UNSPSC: 41115824

Code: PN

Additional Details:
Additional Details: Weight: 1.81440kg



Product Code. 10538723

Quantity Price
1 £ 245.0 / 100mg
EU Stock 78
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Description and Specification

Specification

Chemical Reactivity Amine
Cleavable No
Description EZ-Link Sulfo-NHS-LC-Biotin
Formulation Sulfo-NHS ester of biotin with a 6-aminocaproic acid spacer
Molecular Weight 556.59
Membrane Permeable No
PEGylated No
Spacer Arm Length 22.4
Quantity 100mg
Sufficient For Approx. 50 uses to label a total of 1198mg of IgG using typical conditions
Storage Requirements -20°C with desiccant
Water Soluble Yes

Highlights:

  • Water-soluble analog of NHS-LC-Biotin
  • Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate or phosphate buffers
  • Negative charge of -SO3 group keeps biotinylation localized at the cell surface
  • Creates an amide bond
U.S. Patents 5,892,628 and 5,872,261.

Documentation

Technical documents