Thermo Scientific™ Exonuclease I (Exo I)

Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.

Brand: Thermo Scientific™

Manufacturer Part Number: EN0581

EXO I 20U/UL 4000U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.00900kg

Disclaimers: The purchase of this product allows the purchaser to use it for preparing amplified DNA fragments under a license from GE Healthcare of U.S. Patent Nos. 5,741,676 and 5,756,285 and other foreign patents.

Product Code. 10548930

Quantity Price
1 £61.00 / 4000 units
In Stock 2
EU Stock 125
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Description and Specification


Concentration 20U/µL
Inhibitors 20% (w/v) PEG 8000 (5)
Source E.coli cells with a cloned E.coli sbcB gene
Molecular Weight 54.5kDa monomer
Units 4,000U


  • Active in PCR buffers


  • Primer removal from PCR mixtures:
    • prior to PCR product sequencing
    • li>for one-tube "megaprimer" PCR mutagenesis
  • Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures
  • Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus


The enzyme is not suitable for removing 3'-overhangs of dsDNA.