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Thermo Scientific™ Exonuclease I (Exo I)

Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.

Brand:  Thermo Scientific™ EN0581

UNSPSC : 12352204

Code : 88

Additional Details : Weight : 0.00900kg

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Product Code. 10548930

  • £65.75 / 4000 units


EU Stock 53
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Disclaimers:

The purchase of this product allows the purchaser to use it for preparing amplified DNA fragments under a license from GE Healthcare of U.S. Patent Nos. 5,741,676 and 5,756,285 and other foreign patents.


Description

Description

Highlights

  • Active in PCR buffers

Applications

  • Primer removal from PCR mixtures:
    • prior to PCR product sequencing
    • li>for one-tube "megaprimer" PCR mutagenesis
  • Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures
  • Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus

Note

The enzyme is not suitable for removing 3'-overhangs of dsDNA.

Specifications

Specifications

20U/µL
E.coli cells with a cloned E.coli sbcB gene
4,000U
20% (w/v) PEG 8000 (5)
54.5kDa monomer