Thermo Scientific™ Evolution™ 260 Bio UV-Visible Spectrophotometer

The Thermo Scientific™ Evolution 260 spectrophotometer offers reliable, high-quality performance and enhanced customer usability.

Brand: Thermo Scientific™

Manufacturer Part Number: 840-210900


UNSPSC: 41115406

Code: 04

Additional Details:
Additional Details: Weight: 0.50000kg

Product Code. 10736369

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Description and Specification


Accuracy (Photometric) 1A: ±0.006A
2A: ±0.010A
Measured at 440nm using calibrated neutral density filters traceable to NIST
Baseline Flatness ┬▒0.0010A, 200-800nm, 1.0nm SBW, smoothing
Beam Geometry Double-beam with Application Focused Beam Geometry
Certifications/Compliance ISO 9001:2008
Data Interval 10, 5, 2, 1.0, 0.5, 0.2, 0.1nm
Description Evolution 260 Bio Local Control Spectrophotometer
Detector Type Dual Silicon Photodiodes
Dimensions (L x W x H) 62.2 x 48.6 x 27.9 cm
Display Touchscreen LCD panel; 800 x 480; 17.8 cm (7 in. diagonal)
Drift <0.0005 A/hr, 500 nm, 1.0nm SBW, 1 hr warm-up
Electrical Requirements 100-240 V 50-60 Hz selected automatically 150W maximum
Item Description Evolution 260 LC Spectrophotometer
Keypad Sealed membrane
Lamp Xenon Flash Lamp, 3-year warranty (7 years typical lifetime)
Noise 0A: <0.00015A
1A: <0.00025A
2A: <0.00080A
260nm, 1.0nm SBW, RMS
Operating System Microsoft Windows™ XP embedded
INSIGHT 2 Software is also compatible with Windows 7 and Windows 8.1 Professional Editions and can be installed on a computer attached to the instrument
Optical Design Double Beam with sample and reference cuvette positions; Application Focused Beam Geometry; Czerny-Turner Monochromator
Pharmacopoeia Compliance Testing (Guaranteed Performance Specifications)
Resolution (toluene in Hexane): ≥1.8A
Photometric Accuracy (60mg/L K2Cr2O7): ±0.010A
Stray Light: ≤1%T at 198nm: KCI; ≤0.05%AT at 220nm: Nal, KI
Wavelength Accuracy: ±0.5nm 541.9, 546.1nm Hg emission lines, ±0.8nm full range
Wavelength Repeatability: ≤0.05nm, repetitive scanning of 546.1nm Hg emission line
Photometric Accuracy Instrument 1A: ±0.006A
2A: ±0.010A
Measured at 440nm using calibrated neutral density filters traceable to NIST
Photometric Display -0.3 - 4.0A
Photometric Range >3.5A
Photometric Repeatability ±0.0002A
Range (Photometric) -
Resolution (Toluene/Hexane) -
Scan Ordinate Modes Absorbance, % Transmittance, % Reflectance, Kubelka-Munk, log (1/R), log (Abs), Abs*Factor, Intensity
Scan Speed <1-6000nm/min, variable
Spectral Bandwidth Variable: 1.0nm; 2.0nm; AFBG microcell optimized; AFBG fiber optic optimized; AFBG materials optimized
Type Evolution 260 Bio Local Control Spectrophotometer
Wavelength Accuracy ±0.5nm (541.9, 546.1nm mercury lines)
±0.8nm (full range 190-1100nm)
Wavelength Range 190 - 1,100nm
Wavelength Scan Speed <1 to 6,000 nm/min; variable
Wavelength Slew Speed -
Weight (Metric) 14.4 kg

Complete Life Science Applications

Next generation INSIGHT software provides the streamlined convenience of easy-to-understand nucleic acid and protein analysis methods and includes dedicated modules for:

  • Nucleic Acid concentration by direct UV measurement at 260nm including DNA, ssDNA, RNA, and siRNA
  • Nucleic acid purity measurements from 260/280nm and 260/230 ratios
  • Protein analysis by direct UV measurement at 205 or 280nm
  • Most common colorimetric analysis methods including: Pierce 660nm Protein assay, Bradford, BCA and Lowry
  • Measuring the fluorescence labeling efficiency for proteins and nucleic acids
  • Measuring the microarray labeling efficiency with one or two dyes

Customizable, Straightforward INSIGHT Software

  • Personalize your home screen for easy navigation and convenience
  • Workbook tools save time and ensure accurate results
  • CUE scripting allows creation of customized interface and simplified workflow

Best-in-Class Performance

  • Double-beam geometry ideal for kinetics or any sample that may change over time
  • Application Focused Beam Geometry (AFBG) technology provides superior results for microcells and turbid samples
  • Xenon flash lamp requires no warm-up time allowing instant measurements
  • Electronic triggering provides the highest level of accuracy for rapid-mixing kinetics measurements
  • Precision monochromator drive delivers fast scanning data collection and wavelength accuracy Powerful Convenience at your Fingertips
  • Color, touch screen provides instrument control using built-in computer
  • Integrated keypad allows communication with INSIGHT software
  • Choice of Local or PC configurations provides flexibility and convenience

Accessories for all your Sampling Needs

  • Wide accessory selection with various temperature control options, sample changers, sippers and solid sampling devices complete your analysis
  • Automated cell changers with Peltier temperature control increase sample throughput
  • Choice of Peltier Single Cell Holder or a Smart 8-Cell Peltier system for temperature control and sample monitoring from 0° to 100°C
  • Easy software integration gives you complete control of your measurements

Reliable Assurance of System Performance

  • The Thermo Scientific UV Validator package provides support for system qualification and validation activities
  • Complete documentation facilitates compliance of your system with the requirements of FDA, GxP, ISO 9001:2000 and ISPE 2001 guidelines and regulations
  • CVC and Mercury Lamp accessory automated testing ensures accuracy of your data
  • Thermo Scientific INSIGHTsecurity software provides complete tools to meet 21 CFR Part 11 requirements


Technical documents