ErbB4 (Her4), Biotin, clone: HFR1, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 13-9687-82
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: The monoclonal antibody HFR1 recognizes human and mouse epidermal growth factor receptor 4 (ErbB4 or HER4). ErbB4 belongs to the family of receptor tyrosine kinases (RTK) of which HER1 (EGRF), HER2 (ErbB2), and HER3 (ErbB3) are members. RTKs function to affect a variety of cell processes including proliferation, differentiation, migration, survival and apoptosis and are often disregulated in cancer. The ErbB4 protein contains extracellular ligand binding domains, a transmembrane region, and a cytoplasmic tail containing the tyrosine kinase domain and the binding site for the HFR1 antibody. Upon ligand binding, ErbB4 homo- or hetero-dimerizes with other family members, becomes phosphorylated, and can be cleaved allowing translocation to the nucleus. The HRF1 antibody recognizes membrane-bound, cytoplasmic and nuclear ErbB4. Cytosolic ErbB4 expression was found to correlate with a positive prognosis in a subset of breast cancer patients, while nuclear ErbB4 expression was inversely correlated with tumor grade and aggressiveness of breast tumors.Applications Reported: This HFR1 antibody has been reported for use in immunoprecipitation, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.Applications Tested: This HFR1 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval at less than or equal to 20 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
|Store at 2-8°C. Do not freeze.|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|