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EOMES, eFluor 450, clone: Dan11mag, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-4875-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15519756

  • £210.11 / 100µg


EU Stock 4
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Description

Description

Description: This Dan11mag antibody recognizes Eomesodermin (EOMES), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R.Applications Reported: This Dan11mag antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This Dan11mag antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Buffer Set (cat. 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Eomes
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
O54839
Eomesodermin homolog, EOMES, TBR2
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
Dan11mag
eFluor 450
Eomes
Conjugated
Eomes
IgG2a, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
13813
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