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EOMES, PE-Cyanine7, clone: WD1928, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-4877-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15518556

  • £101.39 / 25 tests


EU Stock 2
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Description

Description

Description: This WD1928 antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R.Applications Reported: This WD1928 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This WD1928 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online.This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

EOMES
Monoclonal
5μl/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
O95936
Eomesodermin homolog, EOMES, TBR2
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
WD1928
PE-Cyanine7
EOMES
Conjugated
EOMES
IgG1, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
8320
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