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EGR2, APC, clone: erongr2, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 17-6691-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15310820

  • £183.67 / 100µg


EU Stock 7
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Description

Description

Description: This erongr2 monoclonal antibody recognizes mouse early growth response 2 (Egr2, Krox-20). Egr2 is a zinc finger-containing transcription factor that is important for the development of T cells and NKT cells. Egr2 is predominantly expressed in the thymus and nervous system, and it is also upregulated in T cells following TCR-crosslinking. Upregulation in all non-adherent mouse splenocytes has been observed following stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin. Egr2 is believed to antagonize T cell activation and has also been reported to promote apoptosis. The erongr2 antibody also appears to modestly stain a subpopulation of T cells and B cells in freshly isolated spleen cells. The erongr2 monoclonal antibody is specific for Egr2 and does not crossreact to Egr3, based on immunoblot analysis.Applications Reported: This erongr2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This erongr2 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523). This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Egr2
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P08152
KROX-20 , early growth response gene 2 , CMT1D , CMT4E , DKFZp686J1957 , FLJ1454
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
erongr2
APC
Egr2
Conjugated
Egr2
IgG2a, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
13654
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