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EBI3 (IL-27 subunit), clone: DNT27, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-7273-82

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15208057

  • £142.50 / 100µg


Estimated Shipment
30-07-2018
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Description

Description

Description: The monoclonal antibody DNT27 reacts with mouse Epstein-Barr Virus-induced gene 3 (EBI3). EBI3 is a 34kDa soluble protein first identified in B cells that is upregulated after Epstein-Barr virus infection. EBI3 can exist as a homodimer and also heterodimerizes with IL-27alpha (p28, IL-30) to form the cytokine IL-27. IL-27 is produced most abundantly by dendritic cells and macrophages with some recent reports of expression in NKT cells. Recently, a complex of EBI3 and the p35 subunit of IL-12, called IL-35, has been reported to be produced by regulatory T cells.Applications Reported: This DNT27 antibody has been reported for use in immunoblotting (WB).Applications Tested: This DNT27 antibody has been tested by western blot of cell lysates prepared from mouse EBI3-transfected 293T cells and bone marrow-derived dendritic cells. This can be used at less than or equal to 1-5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

EBI3 (IL-27 subunit)
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
O35228
IL-27B, IL27B, EBV-induced gene 3 protein, Epstein-Barr virus induced gene 3, IL-27 subunit beta, IL27 subunit, IL35 subunit, cytokine receptor, epstein-Barr virus-induced gene 3 protein, interleukin-27 subunit beta
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Western Blot
DNT27
Unconjugated
Ebi3
Purified
Ebi3
IgG2a, kappa
100μg
Store at 2-8°C.
Primary
50498
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