EBI3 (IL-27 subunit), clone: DNT27, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-7273-82
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The monoclonal antibody DNT27 reacts with mouse Epstein-Barr Virus-induced gene 3 (EBI3). EBI3 is a 34kDa soluble protein first identified in B cells that is upregulated after Epstein-Barr virus infection. EBI3 can exist as a homodimer and also heterodimerizes with IL-27alpha (p28, IL-30) to form the cytokine IL-27. IL-27 is produced most abundantly by dendritic cells and macrophages with some recent reports of expression in NKT cells. Recently, a complex of EBI3 and the p35 subunit of IL-12, called IL-35, has been reported to be produced by regulatory T cells.Applications Reported: This DNT27 antibody has been reported for use in immunoblotting (WB).Applications Tested: This DNT27 antibody has been tested by western blot of cell lysates prepared from mouse EBI3-transfected 293T cells and bone marrow-derived dendritic cells. This can be used at less than or equal to 1-5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|EBI3 (IL-27 subunit)|
|PBS with 0.09% sodium azide; pH 7.2|
|IL-27B, IL27B, EBV-induced gene 3 protein, Epstein-Barr virus induced gene 3, IL-27 subunit beta, IL27 subunit, IL35 subunit, cytokine receptor, epstein-Barr virus-induced gene 3 protein, interleukin-27 subunit beta|
|Store at 2-8°C.|
For Research Use Only.