Thermo Scientific™ DyLight™ Quencher Specialty Dyes

Quench fluorescence resonance energy transfer (FRET) applications involving DyLight Fluors or other fluorescent probes using these non-fluorescent dyes.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 84510

1 MG DYLIGHT 683Q NHS ESTER 1MG STORE IN FREEZERat -20C

UNSPSC: 41116012

Code: PN

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 12311953

Quantity Price
1 £ 220.0 / 1mg
Estimated Shipment date
from Supplier 03-01-2017
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Description and Specification

Specification

Description DyLight 683Q NHS Ester
Formulation Non-fluorescent NHS ester activated Acridinium quencher
Sufficient For 1 use to label a total of 7mg of IgG using typical conditions
Quantity 1mg

DyLight Quencher Specialty Dyes are a family of non-fluorescent dyes designed for fluorescence quenching in FRET applications. The optimal quencher dye can be selected based upon its characteristic absorption properties that match the emission of a corresponding fluor. Each dye contains an amine-reactive NHS ester for simple modification of antibodies, proteins, peptides or other biomolecules through amide bond formation.

Highlights:

  • Large selection– the largest family of dyes available for far red-emitting fluorescence applications

  • NHS ester reactive group–allows immediate labeling of antibodies, proteins, peptides and other amine-containing molecules through amide bond formation

Criteria to consider when choosing a DyLight Quencher Specialty Dye:

  • Absorption wavelength–choose the quencher dye with the absorbance wavelength that matches the emission of your fluorophore and the instrument.
  • Water solubility–choose a dye based on its relative hydrophilicity, which directly correlates to the number of negatively-charged sulfonates it has on its core structure. More hydrophilic dyes are best at maintaining water solubility of a labeled antibody and limiting the nonspecific binding of the conjugate. More hydrophobic dyes often are best at penetrating tissues and cell membranes in vivo, meaning that dyes with fewer sulfonates may work best for some applications.
  • DyLight dye selection–the broad selection of red-emitting dyes allows a number of candidate dyes to be tested in an application for optimal performance.

Recommended for:

FRET; Other quenching applications